抄録
In vitro sensitization, which is considered to be an in vitro model of allograft rejections or G.V.H. reactions in transplantation, was applied for studying human leukemia cell surface antigens.
In this study, patients' peripheral blood lymphocytes (P.B.L.) were co-cultured with their autologous cryopreserved leukemia cells for 7 days and tested onto the autologous 51Cr labeled leukemia cells to see whether the P.B.L. had acquired any cytotoxic functions.
P.B.L. from 17 cases of acute leukemia patients were tested in their complete remission stage and it was observed that P.B.L. from 4 out of 17 patients were stimulated by their own leukemia cells and generated to be cytotoxic cells which were selectively active against their autologous leukemia cells but not to their remission leukocytes.
It was also observed that the P.B.L. from one of 4 positive cases were generated to be cytotoxic cells after co-cultured with solublized cell membrane extract derived from autologous leukemia cells.
