抄録
The monoclonal antibodies of the Coulter clone, T 11, T 4, T 8, B 1, I 2, Mo 2 and J 5 were assesed by indirect immunofluorescent assay using 31 cultured cell lines derived from various type of leukemia, lymphoma and normal B cell lines respectively. Peripheral blood cells including mononuclear cells and polymorpho-nulear cells from five normal adult subjects were also used for the analysis.
The Coulter clone was resulted with high specificity in reaction with human hematopoietic cell line cells and the normal human peripheral blood cells. However, the titer of the monoclonal antibodies were found difference among the cell line cells.
T cell differentiation antigens and functional subsets recognized by T 11, T 4 and T 8 were clearly demonstrated on the cell surface of the T cell lines. However, MT-1 cells derived from the tumor cells of Japanese adult T cell leukemia did not show positive reaction with T 4 and T 8 monoclonal antibodies. CCRF-CEM and RPMI-8402 cells were also negative with T 8 monoclonal antibody. All of the T cell lines were negative with B 1 monoclonal antibody and NALM-6, KOPN-1 and LAZ-221 cells were also negative. In the mature B cell line, Raji cells from Burkitt's lymphoma and U-266 from multiple myeloma were also showed negative with B 1 monoclonal antibody. I 2 monoclonal antibody showed a similar result with the specificity demonstrated by heteroantiserum specific to the Ia-like antigen.
J 5 showed high specificity to CALLA positive cell line cells recognized with anti-CALLA heteroantiserum, CCRF-CEM and NALM-16 cells demonstrated small percentages of positive cells with J 5. However, J 5 is considered to be a specific marker of common acute lymphoblastic leukemia cells including lymphoid precursor cells, Pre T cells, pre B cells and immature B cells.
Mo 2 monoclonal antibody showed specificity reacting with peripheral blood monocytes.
The monoclonal antibodies of the Coulter clone detect the differentiation antigen of human hematopoietic cells and the reagents could be useful for the elucidation for the origin of the malignant cells, the identification of differentiation stage and subset of human hematopoietic cells.
