抄録
Fresh sago palm (Metroxylon sagu Rottb.) leaflets were collected and air-dried. The leaflet samples were heated at 200, 400, and 600 ℃ for the auto-fluorescent analysis (Puppe et al., 2022). The leaflet samples were treated with the wet oxidation method (H2O2 and HNO3 on a hot plate at 80 ℃) to remove organic matter. The phytolith samples were washed with distilled water and air-dried. A part of the phytoliths was dried at 105 ℃ in an oven for a microscope analysis. The phytolith samples were analyzed under a light microscope and a fluorescence microscope. The auto-fluorescence in phytoliths from M. sagu leaflet samples was not observed remarkably. However, the phytoliths in the leaflet samples heated at 200, 400, and 600 ℃ appeared as spotted lumps and strait dotted lines. Heating at 150 ℃ after extraction of M. sagu phytoliths, however, did not show clear auto-fluorescence. It is concluded that 200 ℃ heating might cause auto-fluorescence in organic matter, and both 400 and 600 ℃ heating might increase non-conventional chromophores (oxygen clustering). This study on crucial auto-fluorescence mechanisms in M. sagu phytoliths will open new doors regarding many relatives and fork varieties of M. sagu in South Pacific Ocean islands connected to the birthplace of M. sagu, the Moluccas Islands and New Guinea.
https://ror.org/00qg0kr10 
