Using U-tube electrophoresis it is not easy either to separate certain fraction in sufficient amount or to analyze low molecular substances. To avoid these disadvantages the auther attempted the electrophoresis in an agar gelly column.
Some quantitive analytical results of serum proteins showed good coincidence with those by Tiselius electrophoresis and extaction of certain desired fraction of the protein from agar column was made successfully.
Analysis of human saliva indicated that the amylase of saliva is a single component electrophoretically.
Mixture of certain amino acids in definite amount was analyzed. Results indicated satisfactory separation.
This method may particularlly be available for electrophoretic purification of subjected substance in relatively larger amount.