濾紙泳動法による附加化合物の検出に関する研究III
交叉濾紙泳動法による抗原抗体反応の検出
1959 年 6 巻 1 号 p. 50-57
詳細
1959 年 6 巻 1 号 p. 50-57
By the application of the crossing paper electrophoresis, antigen-antibody reactions could be detected on the filter paper. Antiserum was applied on a line on the anodic side of the filter paper and antigen was applied on a line drawn obliquely to the former on the cathodic side. By the electrophoresis, the antiserum was separated into its components and crossed over by the antigenic proteins. Antigen-antibody precipitates occurred in the zone of γ-globulin of the antiserum.
The line of precipitate formed by the reaction of egg albumin with its homologous antiserum was dissolved and moved, if excess of egg albumin had crossed with it. The antiserum obtained in the earlier stage of immunization, however, showed a line of complex, which was not dissolved by the excess of egg albumin. The complex was suspected not to be precipitate. Thus it was inferred that the anti-egg albumin antibody of the earlier stage would be “incomplete”, which would become complete precipitin afterwards.
The cross reactions of the anti-hen's egg albumin with heterogenous antigens, egg albumins of duck, goose, guinea hen, and quail, could also be deteced.
By the crossing paper electrophoresis of bovine serum and its rabbit antiserum, at least 6 lines of pricipitate were detected. Two-dimensional application of the method was also carried out: The antiserum alone was first separated on a line in the first dimension. Then the antigenic bovine serum was applied on a line vertical to the first one. And the second electrophoresis was carried out to the 2nd direction vertical to the first one. The proteins of bovine serum migrated into the zone of γ-globulin of the antiserum to form lines of precipitate. By the two-dimensional technique, however, the sensibility of the method could not be raised. A comparison with the technique of Garbar's immunoelectrophoresis was made.
The cross reactions of the anti-bovine serum rabbit antiserum with heterogenous antigens, human, swine and goat serum, were detected by the method.
