The effects of sex, strain and age differences and hair plucking on skin enzymes were investigated. The enzymes examined are β-acetylglucosaminidase, β-glucuronidase, β-galactosidase, acid phosphatase, hyaluronidase, (lysosomal enzymes) cytochrome oxidase, monoamine oxidase, isocitrate dehydrogenase, malate dehydrogenase, (mitochondorial enzymes) alkaline phosphatase, 5′-nucleotidase, (membranous enzymes) lactate dehydrogenase, phosphoglucomutase and glucose-6-phosphate dehydrogenase. (cytosomal enzymes) Studies on isozymes were also made for some enzymes.