表面技術
Online ISSN : 1884-3409
Print ISSN : 0915-1869
ISSN-L : 0915-1869
研究論文
チオニン修飾金ナノ粒子を用いた電極表面の機能化と電気化学的グルコースセンサへの応用
安川 智之モハメド カムルル イスラム大橋 拓矢須藤 淳珠玖 仁末永 智一
著者情報
ジャーナル フリー

2005 年 56 巻 10 号 p. 616

詳細
抄録

The electrochemically effective oxidation of NADH has drawn striking attention because it is widely applicable for fabricating biosensors. The mediators are normally used as electron shuttle molecules between the electrode and enzymes. Phenothiazine compounds deserve as suitable mediators due to theirs negative formal potential and large rate constant. For the practical use, both the enzyme and mediator are required to be co-immobilized on the electrode surface. In this study, gold nano-particles modified with thionine (Th+) were immobilized on the surface of the gold electrode by physical adsorption. Th+ was covalently bound to the surface of the gold nano-particles using 3,3'-dithiobis (succinimidyl propionate) (DSP) as a cross linker. The electrochemistry of Th+ modified on the gold nano-particles was characterized by voltammetry. Th+ molecules on the gold nano-particle modified electrodes showed two redox couples at −30mV and 240mV vs Ag/AgCl at pH7. A well-defined peak for the electrocatalytic oxidation of NADH was observed at 240mV. The nano-particle electrodes modified with glucose dehydrogenase (GDH) and Th+ mediators were fabricated and characterized aiming at fabricating a reagentless glucose biosensor. Upon the addition of glucose to the electrochemical cell, the oxidation current dramatically increased due to an electrocatalytic reaction.

著者関連情報
© 2005 一般社団法人 表面技術協会
前の記事
feedback
Top