An experiment for ventilation was carried out in a full-scale test house. This series of studies aims to examine the ventilation measurement method via human expiration. The adopted method is able to measure multi-zone airflow rates. Airflow rate between rooms was controlled and outdoor airflow rate was measured with the test house. The rate of outdoor airflow entering the house and the airflow rate between the rooms were estimated by the ventilation measurement method via artificially provided CO_2 as simulated human expiration based on system identification theory. The estimated results were compared with the measurement results in order to validate its accuracy.