The risk of air pollution derived from the growth of microorganism due to dew condensation in earth-to-air heat exchanger (EAHE) is considered to be a particular problem. In this paper, we predict the thermal performance and dew condensation for an EAHE in an actual building in summer by actual survey and unsteady CFD analysis which did not analyze flow fields to reduce the calculation load. Moreover, we investigate the fungal flora by DNA analysis of fungi sampled in an EAHE.