5 臨床から分離したバイオフィルム形成性Streptococcus intermediusの分子生物学的解析(第514回 大阪歯科学会例会)

抄録

Most bacteria in natural environments form communities, which are commonly referred to as biofilms. Previously, we reported that biofilm formation by oral bacteria have an impact on pathogenesis and plays a key role in development of persistent infections. Using a 96-well microtiter crystal violet (CV) plate assay, we determined the capacity of biofilm-formation of Streptococcus intermedius clinical isolates. The CV analysis showed that glucose increased biofilm-formation of the organism. Furthermore, we isolated the homolog to the pgcA gene, which codes α-phosphoglucomutase in Bacillus subtilis. However, we found that pgcA gene inactivation did not stop biofilm-formation of Streptococcus intermedius.