Studies on Chemical Analysis of Mycotoxins (I)

Gas Chromotography of Patulin and its Derivatives and Gas Chromatographic Analysis of Patulin in Rice

Abstract

Gas chromatographic analysis of patulin was carried out using patulin and its derivatives, trimethylsilyl ether and acetate.
The results showed that in highly contaminated samples, quantitative and qualitative measurements of patulin were possible without making the derivatives, while in lowly contaminated ones, the derivative formation was desirable. Silyl ether of patulin gave the best response in the examined derivatives. The acetate of patulin was sensitive and more than 90% of patulin was acetylated within 4 hours after the addition of reagent. As patulin was markedly colored by the addition of trifluoroacetic anhydride and pyridine, trifluoroacetylation rate could not be tested.
Furthermore, several kinds of solvent systems for the extraction of patulin from rice and the clean up method of the extract for gas chromatography were examined.
Good recovery was obtained by the extraction from rice with the mixture of ethyl acetate and water (71.8%), and then the loss of patulin in clean-up procedure by silica gel chromatography with n-hexane-methanol was negligible.