Studies on Chemical Analysis of Mycotoxins (II)

Gas Chromatography of Penicillic Acid and its Derivatives and Gas Chromatographic Analysis of Penicillic Acid in Rice

Abstract

In the previous papers, the authors reported on gas chromatography of patulin and gas chromatographic analysis on its presence in rice.
In this papers, gas chromatographic analysis of penicillic acid and its derivatives, trimethylsilyl ether, acetate and trifluoroacetate were examined.
Trimethylsilyl ether of penicillic acid was most sensitive among these derivatives, and also the peak on the gas chromatogram was completely separated from that of patulin. Therefore, multidetection of both mycotoxins became possible.
The acetate gave good response and about 50% of penicillic acid unreacted was present after 4 hours of acetylation; however, more than 90% of the acid was acetylated after 24 hours of the reaction.
In comparison with trimethylsilyl ether, trifluoroacetate did not give a desirable response and always gave two peaks on gas chromatogram.
Furthermore, analytical study of penicillic acid in rice was examined. Extraction of penicillic acid from the sample added penicillic acid with the mixture of ethyl acetate and water (7: 1) gave good results and its recovery was 97.1%.