1976 Volume 17 Issue 2 Pages 193-199_1
Systematic analysis for the simultaneous determination of fluorescent mycotoxins, aflatoxin B1, B2, G1, G2, sterigmatocystin, ochratoxin-A and citrinin from polluted grains was studied.
The mycotoxins were extracted with the solvent consisted of 2ml of 20% sulfuric acid, 20ml of 4% potassium chloride solution and 178ml of acetonitrile. They were transfered to chloroform and freed from solvent. By means of silica gel column chromatography the former five toxins were cleaned-up and eluted by chloroform-methanol mixture (97:3) and the remaining ochratoxin-A and citrinin were recovered in the fraction eluted by benzene-acetone-acetic acid (75:20:5) after the intermediate washing of the column with chooroform-methanol (93:7).
Each fraction was concentrated and applied to thin layer chromatography for final determination, in which the mutual separation of ochratoxin-A and citrinin was performed by employing DC-Fertigplatten Kieselgel for solid layer and chloroform-methanol-water-90% formic acid (90:10:1:1) as developing solvent.
The fluorescent intensity of thin layer spots of sterigmatocystin, ochratoxin-A and citrinin were amplified by spraying 20% alminium trichloride solution, exposing in ammonia vaper, and spraying 20% alminium trichloride after exposure in ammonia vaper, respectively.
