農作物中のビペロニルゲトキサイドの分析について (第2報)
高速液体クロマトグラフィーによる定量
1977 年 18 巻 2 号 p. 159-163
詳細
1977 年 18 巻 2 号 p. 159-163
An analytical method of piperonyl butoxide (PB) in cereals by high speed liquid chromatography (HSLC) was investigated.
The HSLC used was “Hitachi 635 type. ” An adsorption column “Hitachi Gel 3010”was employed with ethanol as a mobile phase. The fluorometric detector was set existing wavelength at 290 nm and analyzed at 340nm. The ultraviolet adsorption detector was set at 290nm. Thymol was added as an internal standard.
PB extracted from sample was cleaned up by the partition method using n-hexane and acetonitrile. It was necessary to clean up by the activated charcoal column chromatography in the case of the ultraviolet adsorption detector. PB injected to the column was washed with n-hexane and then eluted with a mixture of n-hexane and chloroform (1: 1).
PB added to samples was recovered in the range from 79.8 to 102%
