In our previous works, we reported many degradation products and reaction products of butyl hydroxy anisol (BHA) with Sodium nitrite or potassium nitrate by irradiation of UV-ray. These compounds were as follows:
Reaction products: 1, 4-dimethoxy-2-nitrobenzene (I), 1-hydroxy-2-tert-butyl-4-methoxy-6-nitrobenzene (II), 1, 4-dimethoxy-2-tert-butyl-6-nitrobenzene (III), 1, 4-dihydroxy-2-tert-butyl-6-nitrobenzene (IV). Degradation products: 2-tert-butyl-quinone (V), 2-tert-butyl-hydroquinone (VI), 2-tert-butyl-1, 4-dimethoxy-benzene (VII), 2′, 3-di-tert-butyl-2-hydroxy-4′, 5-dimethoxy biphenil ether (VIII), 3, 3′-di-tert-butyl-2, 2′-dihydroxy-5, 5′-dimethoxy biphenyl (IX), 3, 3′-di-tert-butyl-2′-hydroxy-2, 5, 5′-trimethoxy biphenyl (X), 3, 3′-di-tert-butyl-2, 2′, 5, 5′-tetramethoxy biphenyl (XI)
In the present paper, we studied on mutagenicity of these compounds. Compound-VI showed a notable mutagenicity in the rec-assay using wild and recombinationless strains of Bacillus subtilis and sensitivity test using wild and rad mutant strains of Yeast in comparison with original materials.
