1983 Volume 24 Issue 5 Pages 484-487_1
A high-performance liquid chromatographic method for identification of oxolinic acid and two major metabolites of piromidic acid, 2-OHPA (8-ethyl-5, 8-dihydro-5-oxo-2-(2-hydroxypyrolidine)-pyrido [2, 3-d]-pyrimidine-6-carboxylic acid) and 3-OHPA (8-ethyl-5, 8-dihydro-5-oxo-2-(3-hydroxypyrolidine)-pyrido [2, 3-d]-pyrimidine-6-carboxylic acid), has been established.
Reversed phase ion-pair chromatography for the separation of these substances was performed on a stainless steel column packed with LiChrosorb RP-18 (10μm), with phosphate buffer (pH 6.4)-methanol (14:11v/v%) containing 0.05% counter-ion (trimethylammonium bromide) as the mobile phase and with detection by absorbance measurement at 330nm.
