1998 Volume 39 Issue 5 Pages 329-332_1
A simple and sensitive method has been developed for the simultaneous determination of six new quinolones (benofloxacin, danofloxacin, difloxacin, enrofloxacin, ofloxacin and orbifloxacin) in beef, pork and chicken.
The drugs were extracted from the meat (1.0g) with 0.3% metaphosphoric acid (2mL) and acetonitrile (20mL). The supernatant was concentrated to dryness after having been defatted with n-hexane (10mL). The residue was dissolved in 5mL of HPLC mobile phase and analyzed by HPLC.
The HPLC analysis was carried out on a TSK-gel ODS 80Ts column (4.6×250mm) with 0.05mol/L phosphate buffer (pH 2.5)-acetonitrile (4:1) containing 0.25g/L sodium octanesulfonate as the mobile phase at a flow rate of 1.0mL/min. A spectrofluorometric detector was operated at Ex 290nm and Em 460nm. The recoveries of six drugs from fortified meat were greater than 80% and the detection limits were in the range of 0.0025-0.05μg/g.
This method is concluded to be useful as a simultaneous analytical method for new quinolones in meats.
