1998 Volume 39 Issue 5 Pages 333-340_1
A rapid and highly sensitive method has been developed for the simultaneous determination of reducing monosaccharides and oligosaccharides by normal-phase HPLC with fluore-scence detection. The technique of linear gradient elution on a TSKgel Amide-80 column was more suitable for saccharides separation. The post-column reaction was optimized for fluorometric detection. Under optimum conditions, the detection limits were 0.3-15ng for the reducing saccharides investigated. The calibration curves were approximately linear in the range of 2, 000-12.5pmol for glucose, cellotriose, and cellopentaose. The coefficient of variation for glucose was less than 1%. Application of the method for food analysis was successful.
