1968 年 9 巻 1 号 p. 40-44
The present experiment was aimed at separating EDTA by ion exchange resin method from free amino acids in foods which interfere with the color reaction of EDTA with metal ions based on complex production.
By filtration through weak alkaline anion exchange resin column adjusted to pH 2.1±0.1, EDTA was adsorbed on the resin column and interferring amino acids passed through it.
The adsorbed EDTA was eluted by 2N HCl. Typical results by this method are given in Fig. 2.
Detectable limit of EDTA by the combination of this separation method and the color reaction with cobalt ion and hydrogen peroxide was about 0.4mg.