抄録
High throughput microchip assays for NO metabolites and catecholamines in human body fluids were investigated, which have several advantages for human-fluids monitoring. In NO metabolite assay, we fabricated quartz microchips using laser beam direct draw (LBDD) and developed an advanced microfluidic control to realize high throughput assay. The NO metabolites were completely separated within ca. 15 seconds. In catecholamines (CAs) assay, we fabricated plastic microchips using LIGA process and developed a method to analyze CAs. The CAs were separated within 2 minutes. We also applied a human saliva before/after the tension-stress model. We demonstrated the stress marker assay using microchip technology at the first time as far as we know.
