Roost usage during the hibernation by cave-dwelling bats was studied in Kochi Prefecture from 2003 to 2005. The following four species were found from the roosts: Rhinolophus ferrumequinum, Rhinolophus cornutus, Myotis macrodactylus and Miniopterus fuliginosus. The number of roosts used by bats was highest in R. ferrumequinum, though R. cornulus was most abundant among the four species. Although the hibernation colony of M. fuliginosus in the Ryugado cave had been reported, the occurrence of this colony was not confirmed in the present study.