A Method of Observation of the Embryonic Development of Medaka Fish Using Poster-adhesive Stickers

Abstract

We conceived of a method using poster-adhesive stickers (such as the brand Wappon) for the observation of eggs and embryos of Medaka fish (Oryzias latipes) under microscope. Two pieces of Wappon are put on a slide glass, leaving about 1 cm of space between them, and a Medaka egg with water is placed in the space between the two Wappon, and another cover glass is placed on top of the egg, creating a bridge over the two Wappon. The thickness of the Wappon is slightly thinner than the diameter of Medaka egg, so the covering glass puts some pressure on the egg. By sliding the cover glass on the Wappon, the students can rotate the egg and change direction, and the student can obtain clear microscopic images. The material cost is inexpensive, and the way of making the slides is not too difficult for children. This method can be utilized in science or biology lessons in elementary school, junior and senior high schools.