抄録
A sensitive and simple analytical system for the determination of 1-deoxynojirimycin (DNJ) was established using hydrophilic interaction liquid chromatography (HILIC) with tris（2,2'-bipyridine）ruthenium (II) - electrogenerated chemiluminescence detection. Chromatographic separation of DNJ was achieved on a TSKgel Amide-80 column (5 μm, 250 x 4.6 mm id). The mobile phase was an 82:18 (v/v) mixture of acetonitrile and 5 mM KH2PO4 buffer (pH 5.8), flowing at 0.9 mL/min. In addition, 10 mM KH2PO4 buffer (pH 7.5) was mixed at 0.3 mL/min post column to raise the pH of the mobile phase to the optimal pH for detection. A 1.5 mM H2SO4 solution containing 0.3 mM tris（2,2'-bipyridine）ruthenium (II) chloride was used as the detection reagent solution, which was circulated at 0.4 mL/min through electrochemiluminescence detector (COMET 3000) controlled to 80 μA oxidation current. These optimized conditions were used for quantitative analysis of DNJ. The detection and quantification limits of DNJ were 0.1 and 0.15 μg/mL, respectively, and the calibration curve showed good linearity (r 2 > 0.9999) in the range of 0.15-20 μg/mL. The recovery rate of DNJ from the mulberry leaves samples was more than 97 ％. Moreover, the DNJ content of five mulberry leaf tea products was determined using this analytical system and was found to vary between 0.03 ％ and 0.21 ％ depending on the product. These results demonstrate the applicability of this analytical system for quantitative analysis of DNJ in mulberry leaves, and its promising potential for application in marine bioprospecting.
