Effect of Reaction Conditions on L-Histidine Decarboxylation Activity of Halophilic Histamine-Forming Bacteria
1993 Volume 59 Issue 10 Pages 1745-1748
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1993 Volume 59 Issue 10 Pages 1745-1748
the effect of reaction conditions on L-histidine decarboxylation activity of halophilic histamine-forming bacteria (Photobacterium histaminum (C-8) and P. phosphoreum (N-14)) were studied and the results were as follows.
The opitimum temperature and pH value for the decarboxylation activity of C-8 were 35°C and 6.0, respectively, while those for N-14 were 30 ?? 35°C and 6.0, respectively. The Km value for C-8 (3.70mM) was 5 times smaller than that for N-14 (18.8mM), showing a powerful affinity for histidine. The presence of histamine showed an inhibitory effect for the histidine decarboxylation activity of C-8, while agmatine and putrescine besides histamine did so for N-14.
