Effects of Storage Conditions on Electrophoregrams of Proteins and LDH Isozymes from Skeletal Muscle and Vitreous Humor of Ocellate Puffer

Abstract

The proteins in skeletal muscle extract and vitreous humor from Takifugu rubripes were separated by polyacrylamide gel isoelectric focusing (PAG-IEF), and their L-lactate dehydrogenase (LDH) isozymes were separated by agarose gel electrophoresis.
The electrophoregrams of proteins and LDH isozymes showed specific patterns for each of skeletal muscle and vitreous humor. These patterns were not significantly different among 10 specimens of pufferfish. The protein patterns of skeletal muscle were changed by storage at 4°C, but not at all by storage at -20°C for 24 weeks. The protein patterns of vitreous humor were markedly changed by storage even at -20°C. However, the LDH zymogram of vitreous humor was not significantly changed by storage at 4°C for 1 week or at -20°C for 24 weeks.
Thus the electrophoretic analyses of skeletal muscle proteins and of LDH isozymes from skeletal muscle and vitreous humor of pufferfish are assumed to be useful for the distinction of pufferfish species even after storage at -20°C for 24 weeks.