抄録
The biotransformation of the foreign substrate using the living cells is interested in medical, pharmaceutical and organic synthesis fields. In such a status we had studied the biotransformation of foreign substrate using plant cultured cells and it was found that the plant cells have the conversion ability such as stereoselective reduction, enantioselective oxidation, regioselective hydroxylation and glycosylation. the saponine is accumulated in plants as secondary metabolites and it's ability in high functionally and draw an international attention as functional material. Also glycosylation was useful primarily for enhancement of water-solubility and thermal stability. We had investigated the production of saponines in one step and at reasonably low cost by using plant cultured cells. In this way, we have studied the biotransformation of using plant cultured cells. Recently we extract glucose transferase (PaGT3) derived from plant cells. To synthesize of the high functional saponines using PaGT3 we study the biotransformation of substrate using PaGT3 enzyme. To produce the glycoconjugates efficiently, we could use not only the purified enzyme but also the recombinant E.coli cells containing the PaGT3 gene. At this time we use trans-resveratrol as a substrate.The enzyme reaction trans-resveratrol→trans-resveratrol 4'-0-13-D-glucoside (yield 2.5%) The recombinant E.coli cell cultures reaction trans-resveratrol→trans-resveratrol 4'-0-13-D-glucoside (yield 11.7%) From this result it was found that the plant cultured cells and PaGT3 glycosylate the hydroxyl group of phenols in a good yield.
