The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Culture Conditions Necessary to Sustain the Serial Culture of Lung Cells of Newborn Hamster in Albumin Fortified Medium
YUTAKA MATSUYAISAO YAMANE
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1970 Volume 101 Issue 4 Pages 387-396

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Abstract

Using various albumin fortified medium, many neonatal hamster lung cells were developed into established lines. The optimal culture condition to evolve into cell lines was investigated by varying the source of albumin, concentration of serum, inoculum density, medium component and time interval. Also, the minimum culture condition necessary to sustain the serial culture was examined. The requisite in the culture condition for the development of established cell lines was discussed.

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