Abstract
In the course of cell establishment, neonatal hamster lung cells progressively acquired various new properties and they showed morphological and karyological changes. The cells became capable of growing infinitely in albumin-free medium and these cells also had an ability to grow at low inoculation densities. The neoplastic property of the cells developed more slowly than their chromosomal alteration into the heteroploid range. The established line could similarly propagate in both the standard medium and serum albumin medium, and consequently the growth promotion by bovine albumin became almost negligible.
