Abstract
Two cell lines of D cells derived from the same population of lung cells of newborn hamster were serially cultivated in media fortified with different kinds of albumin (bovine serum albumin and egg albumin) and were eventually developed into established lines. On the cultures carried in egg albumin medium, however, serum albumin became gradually growth-inhibitory, while the cells grown in serum albumin medium were not influenced by supplement of either serum albumin or egg albumin to the medium. That the two established lines had different cellular properties was confirmed by morphological and karyotype analysis.
