1971 Volume 103 Issue 2 Pages 195-201
The splenic subeellular fraction which was effective in prolonging the survival time of rats with methylcholanthrene-induced sarcomas (recipients) was prepared by the following procedure. The supernate of each recipient's tumor homogenate emulsified in sucrose solution was obtained by ultra-centrifugation (5000×g) at 4°. After injections of tumor-supernate into donor rats, the spleens of donors were removed. The supernate of the splenic cells was prepared by means of the same procedure as the supernate of tumor. The recipients, which were treated with the splenic supernate immunologically sensitized with recipient's own tumor, survived longer than control rats which were treated with the splenic supernate sensitized with tumor of the other allogeneic rats.
