The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Identification of Individual Acid Mucopolysaccharide in Tissue Sections
YOICHIRO SASAI
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1971 Volume 105 Issue 2 Pages 101-110

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Abstract
Streptomyces hyaluronidase and chondroitinase ABC were used for the identification of individual acid mucopolysaccharide in tissue sections. Materials used were as follows; cocks comb, femur-tibial joints, costal cartilage and tongue of rat, human umbilical cord and human skin. Staining was carried out with alcian blue containing electrolytes of different concentrations according to the critical electrolyte concentration concept. The results observed suggest that streptomyces hyaluronidase and chondroitinase ABC are applicable to tissue sections for the identification of acid mucopolysaccharides.
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© Tohoku University Medical Press
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