The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Volume 105, Issue 2
Displaying 1-12 of 12 articles from this issue
  • YOICHIRO SASAI
    1971 Volume 105 Issue 2 Pages 101-110
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Streptomyces hyaluronidase and chondroitinase ABC were used for the identification of individual acid mucopolysaccharide in tissue sections. Materials used were as follows; cocks comb, femur-tibial joints, costal cartilage and tongue of rat, human umbilical cord and human skin. Staining was carried out with alcian blue containing electrolytes of different concentrations according to the critical electrolyte concentration concept. The results observed suggest that streptomyces hyaluronidase and chondroitinase ABC are applicable to tissue sections for the identification of acid mucopolysaccharides.
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  • TADANOBU FUJIYAMA, RYUJI YAMAGUCHI, KIICHIRO NODA
    1971 Volume 105 Issue 2 Pages 111-121
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    The placenta and umbilical cord are conceived in general to have no nerves, while their blood vessels are experimentally known to contract or dilate in response to certain drugs or specific conditions. To explain this relation, the authors, in the present study, examined the presence of nerve fibers in the human umbilical cord and placenta by using Seto's silver impregnation staining. The results were: (1) Nerve fibers, stained by the silver impregnation, were found in the umbilical cord, existing in the form of nerve bundles mainly in the Wharton's jelly. Fine fibrils that diverged from the bundles partly formed a- terminal reticulum or freely ended in the vascular wall. (2) The nerve was amyelic, morphologically classified as the vegetative nerves. (3) The nerves in the umbilical cord were present limitedly in the 1/3 to 1/4 range proximal to the fetus, which indicated that they had probably extended by way of the umbilical ring from the fetus. (4) No nerve fibers were found in the placenta.
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  • TOORU NAKAO, SHUNZO CHIBA, KIYOTAKA HORINO, YASUO CHIBA
    1971 Volume 105 Issue 2 Pages 123-127
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Three cases of paralytic disease associated with poliovirus infection were reported. One patient had not received poliovirus vaccine nor had any contact with an individual who had received vaccine. Poliovirus type 1 was isolated from feces of this patient, and virologic studies revealed that this strain was probably a wild-type. Two patients had received the trivalent oral poliomyelitis vaccine before onset of illness. No virus was isolated from one patient, but neutralizing antibody titers against three types of polioviruses were risen. Poliovirus type 2 was isolated from feces of the other patient, and this strain was found to be vaccine-like. The fact that there is wildtype of poliovirus in spite of mass immunization against poliomyelitis, indicates the possiblity of outbreak of poliomyelitis when immunization rate falls under critical level.
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  • RYUJI YAMAGUCHI, KIICHIRO NODA, TADANOBU FUJIYAMA
    1971 Volume 105 Issue 2 Pages 129-135
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Alkaline phosphatase (AP) activity in the placenta was investigated histochemically in relation to the characteristic patterns of the heat-stable alkaline phosphatase (HSAP) in the serum of near-term women, which were classified in the previous report into four, i.e., elevating, descending, flattening (plateau), and fluctuating (unstable). Most of the women who appared entirely normal and showed either elevating or descending pattern in their HSAP activity, maintained a steady activity of AP in the chorionic epithelium of the placenta. On the contrary, most of the cases which are conceived as abnormal and showed fluctuating pattern in the HSAP activity revealed a decreased activity of AP in chorionic epithelium. Such decrease in the placental AP activity was observed predominantly in postdated women with the fluctuating pattern, but such relationship between AP activity in the placenta and the pattern of HSAP was less evident in cases with pregnancy toxemia.
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  • MASAFUMI SHIRAI, MAMORU NAKAMURA
    1971 Volume 105 Issue 2 Pages 137-140
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    113mIn-microcolloid which has an extremely short half-life and is able to administer a large dose, and of which urinary excretion and retention in the circulation are low, was used in radioisotope penogram. Much higher count values were obtained than in the penogram using the conventional 131I-human serum albumin, so that the differential diagnosis between functional and organic impotence became easier.
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  • KUN-YOUNG KANG, SADAO OHIRA, TATUO SAITO, AKIRA NAGAI, YUTAKA MIURA
    1971 Volume 105 Issue 2 Pages 141-146
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Ultracentrifugal separation and analysis of chylomicron, very low, low and high density lipoproteins in the sera from patients with liver metastasis of cancer were carried out. Studies were made on the relationship between lipoprotein and alkaline phosphatase (Al-P) on cellogel electrophoresis. 1) On cellogel electrophoresis, the serum Al-P isozyme of a patient with liver metastasis was divided into Bands I and II. Band I which corresponds to macromolecular Al-P is characteristic of liver metastasis. 2) Band I was found only in high density lipoprotein (HDL) fraction. This result indicates that the density of Band I is very close to that of HDL. 3) The electrophoretic mobility of macromolecular Al-P was the same with that of HDL. 4) No relationship was found between Band II which corresponds to Al-P with a small molecular weight and lipoprotein. 5) By treatment with receptor destroying enzyme of the serum, the electrophoretic mobility of the lipoproteins remained unchanged. Band I, however, was detached from HDL and moved toward the origin. These results suggest that Al-P bands contain a large quantity of sialic acid.
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  • NOBUKAZU CHIDA, TOSHIHIRO OKAMURA
    1971 Volume 105 Issue 2 Pages 147-155
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    In two cases of idiopathic hypercholesterolemia, aged 6 and 12 years, it was found that in vivo incorporation of sodium acetate-1-14C into serum cholesterol was markedly increased, but that into squalene and lanosterol in serum was less than that in controls. In vitro incorporation of sodium acetate-3H or DL-mevalonic acid-2-14C into cholesterol was markedly increased in the liver homogenates from a patient with hypercholesterolemia, but it showed no difference between the patient and controls when skin, adipose tissue, bone marrow cells or leukocytes were used.
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  • TOSHIMITSU KOTOKU, HIROSHI KAWAKAMI, TAKAYUKI IWABUCHI, TSUTOMU SATO, ...
    1971 Volume 105 Issue 2 Pages 167-175
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Serum creatine phosphokinase (CPK) activities in 104 patients with cerebrovascular diseases were estimated and their clinical significance was discussed. Thirty-eight of 54 patients at acute stage showed the serum enzyme levels of over 100 I. U. per liter, though those of all 50 patients at chronic stage showed normal values. Serum CPK levels higher than 300 I. U. per liter were found in 11 cases, of which 4 revealed relatively good prognoses, so that high CPK activity would not always signify a severe clinical status or a poor prognosis. On the other hand, the outlook over the patient whose serum CPK level has been within normal range during the first 2 or 3 days after the onset might be favorable. Comparison of CPK activity in the sera from the jugular vein, peripheral limb vein and femoral artery failed to disclose the source of plasma CPK activity increased in the acute CVD.
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  • YUKIO KIMURA
    1971 Volume 105 Issue 2 Pages 177-190
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Effects of section and stimulation of the hypogastric, pudendal and pelvic nerves on emission of the seminal fluid into the posterior urethra and on ejaculation from there were observed recording the posterior urethral pressure, using mongrel dogs. Bipolar electric stimulation was given continuously to the nerves under the conditions of 0.5-2V, 0.5-2 msec, and 5-40 cps. For recording the posterior urethral pressure, a #6 polyethylene tube was inserted into the posterior urethra. On recording, communication between the posterior urethra and the urinary bladder was blocked to prevent regurgitation of the seminal fluid into the bladder and the external urethral orifice was ligated to produce a similar effect to an increased resistance in the anterior urethra by erection. The results obtained were as follows: Among the above-mentioned nerves, emission occurred by stimulation of only the hypogastric nerve. The emission caused a gradual rise in the posterior urethral pressure. When it reached the maximum, rhythmic alterations occurred. The rhythmic alterations were concluded to be the same phenomenon as ejaculation. It was noted that for occurrence of rhythmic alterations, both the hypogastric nerve and the pudendal nerve were concerned and that the pelvic nerve as well as the hypogastric nerve was involved in seminal emission.
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  • TOORU NAKAO, SHUNZO CHIBA, MASAMI OHSAKI, SHIGERU IKEDA
    1971 Volume 105 Issue 2 Pages 191-195
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Histological examinations of post mortem specimens, virus isolation and the indirect immunofluorescent test for cytomegalovirus macroglobulin were carried out in newborn infants. The most reliable method for a diagnosis of congenital cytomegalovirus infection in newborn infants was virus isolation, but the detection of cytomegalovirus IgM antibody by indirect immunofluorescent method could be used for a diagnosis of asymptomatic cytomegalovirus infection.
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  • HACHIRO INOMATA, TAIZO SUZUKI
    1971 Volume 105 Issue 2 Pages 197-198
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    Spontaneous junctional potentials (SJP) in guinea-pig vas deferens were investigated with intracellular microelectrodes. In some of the cells examined, SJP was atropine-sensitive and presumably cholinergic in nature. This finding stands in contrast to the generally accepted concept that all of SJPs in vas deferens are atropine-resistant and noradrenergic in nature. It seems more likely from this result that there exist two types of SJPs in vas deferens; one is noradrenergic type and the other is cholinergic type.
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  • AKIRA RIKIMARU, YASUE FUKUSHI, TAIZO SUZUKI
    1971 Volume 105 Issue 2 Pages 199-200
    Published: 1971
    Released on J-STAGE: November 28, 2008
    JOURNAL FREE ACCESS
    The effects of imidazole and phentolamine on the relaxant responses of the isolated guinea-pig taenia coli to transrural stimulation (1 msec, 5 pulses/sec) and to ATP (10-6-10-5g/ml) were studied. The relaxation produced by ATP was entirely abolished by imidazole (3.5×10-3g/ml) or phentolamine (5×10-5g/ml), though the relaxation due to transmural stimulation remained unimpaired. These results are rather controversial to the concept that the transmitter substance of the non-adrenergic inhibitory nerves in the gastrointestinal tract is ATP.
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