Abstract
Carbonic anhydrase (CA) of human red cells, especially of newborns, was quantitated by Ascoli's ring test. The anti-CA B serum was obtained from a rabbit immunized with pure CA B prepared by column chromatography, and the antiserum reacted with CA B, CA A and another isozyme of adult blood. Only CA B was demonstrated in the cord blood by the antiserum. CA B amounts in the cord blood hemolysates (142 cases) ranged from 0.0625 to 2mg/ml of hemolysate containing 36g hemoglobin per 100ml. The mean was 0.841 (S. D. 0.393)mg/ml of the hemolysate. The average of CA amount of the adult blood hemolysates (23 cases) including a small amount of the other isozymes was 7.96 (S. D. 0.528)mg/ml. The average of CA B amounts of 4 premature infants was 0.083mg/ml. Fetal hemoglobin (HbF) measured by one minute alkali-denaturation was 70.7 (S. D. 6.64) % in 116 cord bloods, and CA B amount of the bloods was inversely related to HbF percentage. It is presumed that HbF gene is probably an antagonistic control of CA B gene.
