The Localisation of a Lesion in the Brain by Differential Staining of Blood Smears

Third and Fifth* Reports of the Same Title 18th Report of the Peroxidase Reaction

Abstract

In an attempt to make the “peroxidase puncture” in 223 rabbits with a modified technique I was able to produce the “striatal blood picture” in 7 animals. The small number of the successful “peroxidase puncture” shows the difficulty of the operation even with the modified technique.
1. Those successfully “pcroxidase punctured” animal develop the “striatal blood picture” from 1_??_to 24 hours after the puncture.
2. As Sato has already suggested in his first report, it is only the myeloid leucocyte in the streaming blood that will become peroxidase-ncgative, while they remain oxidase-positive. The leucocyte of the same category in the bone marrow, for instance, remains oxidasc-as well as peroxidase-positive.
3. The smallest lesion with the “peroxidase puncture” extends as far (cranial) as the foremost part of Aquaeductus Sylvii and includes in it the part near oculomotor nuclei. As to the above limit Sato has already shown that it does not surpass the level of the Aquaeductus Sylvii.