Correlation between Skin-Lymphocyte Culture Test (SLC Test) and Skin Allograft Survival in Mice

Abstract

SLC test with the mixed culture of the donor's skin and recipient's lymphocytes, was investigated for tissue typing in mice. Mice used were C₃H/He (H-2^k), CBA (H-2^k) and (C₃H/He × C₅₇BL/6) F₁. SLC test was carried out with a stationary monolayer culture in Eagle's basal medium containing 0.25% phytohemagglutinin-P for 72 hours. The extent of the reaction was estimated by both the frequency of the appearance of blast-like cells on morphological observation, and the number of the aggregated lymphocytes surrounding the skin in Ehrlich's sight. The extent of the blast formation and aggregation of lymphocytes was compared with the survival time of the skin grafts in various combinations of donors and recipients. A significant correlation between the result of SLC test and the transplant survival time was found.