Abstract
The activity of lecithin formation from lysolecithin (lysolecithin: lysolecithin acyl-transferase) in 105000 xg supernatant fraction of various rat tissues was deter-mined with lysolecithin labeled with 3H-glycerol as substrate. The highest values of the activity (n moles/mg protein/min.) found in supernatant fractions in lung, intestine, liver and spleen were 9, 8, 5.7, 4.9 and 2.3, respectively. The activity was mainly localized in the supernatant fraction in spleen and in lung but it was rather lower in the supernatant fraction than in the particulate fraction in intestine and in liver. These tissues also showed a high activity of lysolecithinase in the following descending order; intestine, lung, liver and spleen. Low activiti-es of both enzymes were found in four tissues; heart, skeletal muscle, kidney and brain.