Effect of Diphenylhydantoin Administration on Single Carbon Metabolism in Folate Deficient Rats

Abstract

When rats were made folate deficient and given diphenylhydantoin (DPH), it was found that there were an increase in formation of ¹⁴CO₂ from urocanate-2-¹⁴C and histidine-2-¹⁴C by liver slices, a decrease in urinary formiminoglutamic acid (FIGLU) after urocanate load, and an increase in the incorporation of formate-¹⁴C into serine by liver slices. These results suggested that utilization of single carbon from FIGLU and formate via folate pathway proceeded more effectively in folate deficient rats when DPH had been administered.