Abstract
A new technique for determining causative agents in various sites of air way in patients with respiratory infection was devised in which sputum specimens were directly aspirated from various sites of air way by use of the Kifa-Green catheter and each of them was cultured for the determination of causative agents. This method has an advantage for obtaining information of an exact location of causative agents in air way. Main causative agents detected by this method in patients with various respiratory infection were Haemophilus influenzae, Pseudomonas aeruginosa, and Diplococcus pneumoniae.