Thin Layer Chromatography and Fluorescent Scanning Analysis of Porphyrins

Abstract

A combined technique of two dimensional thin layer chromatography with fluorescent scanning was applied to the estimation of urinary porphyrins in 2 patients with porphyria cutanea tarda. Porphyrin patterns were also examined with the technique on urine, liver and skin samples obtained from a hexachloro-benzene-induced porphyric rat. Results obtained showed that uroporphyrin and hepta-carboxylic porphyrin were predominant in the urine, liver and skin.