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Volume 121 (1977)
- Issue 4 Pages 301-
- Issue 3 Pages 197-
- Issue 2 Pages 99-
- Issue 1 Pages 1-
Volume 121, Issue 1
Displaying 1-13 of 13 articles from this issue
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YOICHIRO SASAI, SHOZO NAKANO, KENJI ANDO1977 Volume 121 Issue 1 Pages 1-12
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSGlucose-6-phosphate dehydrogenase activity was studied in hair follicles from both the bald and hairy regions of the scalp of 5 patients with male pattern alopecia by the application of the method of Lineweaver-Burk to the histochemistry and by the fluorometric method of Lowry. In vitro experi-ment showed that the incubation time necessary for yielding a certain amount of formazan is related to the amount of enzyme present. In the case of section experiment, the time required for the first appearance of formazan deposition in the tissue at various substrate concentrations was plotted against the reciprocal of the substrate concentration. The data obtained by this method seem to be consistent with the data by the fluorometric method.View full abstractDownload PDF (714K) -
YASUNOBU HANDA1977 Volume 121 Issue 1 Pages 13-25
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSMorphological changes of partially dissociated chick spinal ganglion cells in culture and their intracellular responses were simultaneously observed in an attempt to investigate the correlation between cell structure and function during development in vitro. As the ganglion cells matured, their originally eccentric nuclei assumed more central positions and the surface area of cell body displayed a linear increase accompanied with an increase in cell diameter. Nissl bodies were distributed throughout the cytoplasm after 3 to 4 weeks and myelin was formed at the same period. The bioelectric properties of the ganglion cells changed in parallel with the morphological development. The passive membrane properties such as resting potentials and specific membrane resistances were increased. Action potentials could be elicited from neurons as early as 2 days in vitro, while the complete excitability was accomplished at 8 to 10 days. Moreover, the wave form of the action potential itself was changed, that is, the amplitude and maximum rate of rise increased and the duration decreased. The alteration rates of these passive and active properties were maximum at 8 to 12 days in culture. Repetitive firing occurred after 3 to 4 weeks, though single responses were displayed by 3 weeks in culture. This changes seemed to correspond with myelin formation in vitro.View full abstractDownload PDF (2194K) -
Insulin Response to Glucose or Glucagon in Subclinical Diabetes Previously Injected with TolbutamideAKIRA OHNEDA, KIYOSHI WATANABE, YOSHISUKE MARUHAMA, HIROSHI ITABASHI, ...1977 Volume 121 Issue 1 Pages 27-32
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThirty-one patients with subclinical diabetes, who showed diabetic or impaired glucose tolerance after treatment for diabetes, were investigated in order to clarify the abnormalities of insulin response in diabetes mellitus. These patients showed a delayed response of plasma insulin during oral glucose loading. In the tolbutamide-glucose test, in which glucose loading followed the intravenous tolbutamide injection at a 60-min interval, the insulin level at 90 min was significantly lowered in a group of 20 patients with subclinical diabetes. In the tolbutamide-glucagon test, in which 1 mg of glucagon was injected 60 min after tolbutamide injection, the maximal level of plasma insulin was significantly decreased in a group of 10 subclinical diabetics except for one patient. These results indicate that insulinogenesis and/or release of insulin were decreased even in subclinical diabetes, suggesting that such a defect in islet function might be one of the abnormalities in primary diabetes.View full abstractDownload PDF (345K) -
YOSHINOBU ISHIKAWA, AKIRA HOKAMA, MATSURO FUKUSHIMA, TEIJI NISHIKAWA, ...1977 Volume 121 Issue 1 Pages 33-39
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe influence of physical and chemical treatments upon the immunologi-cally effective subcellular fractions (5, 000×g supernatant and 105, 000×g sediment), which were prepared from the spleen cells of Yoshida sarcoma (YS)-resistant Donryu rats, was studied. The immunological activity of the 5, 000×g supernatant was stable to heating at 80°C for 30 min. It was stable to alkali (pH 10) and less stable to acid (pH 2). It was labile to a ten-day storage at 4°C, but relatively stable to a 30-day storage at -20°C. When the 105, 000×g sedi-ment was lyophilized and stored at -20°C for 95 days, its immunological activity was well maintained. It was labile to 95% ethanol, 90% phenol and 2 M NaNO2, but relatively stable to 10% as well as 100% acetone, 10% phenol and 10% ethanol. It was labile to 0.1 M NaIO4 and relatively stable to 0.1M K2Cr2O7 solution. It was labile to RNase but relatively stable to DNase.View full abstractDownload PDF (408K) -
OTOTAKA HIGASHI, WAKAKO ISAIGAKI1977 Volume 121 Issue 1 Pages 41-46
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSHydrogen peroxide can either induce or inhibit or enhance the platelet aggregation in vitro depending upon the experimental conditions. Vitamin E and Vitamin E-nicotinate are found to be effective to inhibit, to some extent, the platelet aggregation induced by combined adenosine diphosphate (ADP) and hydrogen peroxide (H2O2), added simultaneously, to the platelet rich plasma (PRP). Vitamin E and Vitamin E-nicotinate seemed, however, to be unable to prevent the reduction of platelet response to ADP, which was brought about by the pretreatment of PRP with H2O2 of a lower concentration.View full abstractDownload PDF (326K) -
TETSU NAGATA, YASUMASA HAYASHI1977 Volume 121 Issue 1 Pages 47-56
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn-fluences of saccadic eye movements upon excitability of superior colliculus (SC) were examined in chronic cats carrying implanted electrodes and in encéphale isolé cats. Excitability of SC was assessed by recording responses of SC to electrical stimulation of cortico-collicular (CC) pathway. The SC response to stimulation of CC fibers consisted of two components, i. e., presynaptic T and postsynaptic R. Eye movements enhanced R but did little change T. Enhancement of the postsynaptic component R was ascribable to a central factor on the basis of the following results: 1) Enhancement of R occurred independently of whether the ambient illumination was present or not. 2) Saccade-like movements of a slit of light in front of immobilized eyes never induced enhancement of R. 3) PRF stimulation, effective to cause saccade-like eye movements, always elicited enhancement of R, independent of the ambient condition of eyes and even after eyes had been immobilized.View full abstractDownload PDF (600K) -
TAKASAI MIURA, WAKIO TORINUKI1977 Volume 121 Issue 1 Pages 57-61
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA combined technique of two dimensional thin layer chromatography with fluorescent scanning was applied to the estimation of urinary porphyrins in 2 patients with porphyria cutanea tarda. Porphyrin patterns were also examined with the technique on urine, liver and skin samples obtained from a hexachloro-benzene-induced porphyric rat. Results obtained showed that uroporphyrin and hepta-carboxylic porphyrin were predominant in the urine, liver and skin.View full abstractDownload PDF (291K) -
TETSURO NISHIHIRA, TAKAO KUNORI, MASAKI TAN, EIAKI TSUTSUMI, MORIO KAS ...1977 Volume 121 Issue 1 Pages 63-68
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA highly specific anti-human T lymphocyte serum (ARTS) was obtained by sequentially absorbing rabbit anti-human thymocyte serum (ATS) with human red cells, neutrophils, Burkitt's lymphoma cells (Daudi cells, ) liver, kidney and glutaraldehyde insolubilized human serum. The immunofluorescent staining revealed that the basement membrane of human renal glomeruli reacts with ARTS before being absorbed with kidney. Specificities of ARTS were studied by the cytotoxicity test, inhibition of PHA responses, inhibition of E-rosetting and immunofluorescent staining.View full abstractDownload PDF (860K) -
TETSURO NISHIHIRA, TAKAO KUNORI, MASAKI TAN, ETAKI TSUTSUMI, MORIO KAS ...1977 Volume 121 Issue 1 Pages 69-75
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAnti-human B lymphocyte serum (AHBS) was obtained by absorbing rabbit anti-human tonsil serum (ATOS) or rabbit anti-human spleen serum (ASPS) with human red cells, liver, neutrophils, thymocytes and glutaraldehyde insolubilized human serum. Specificities of AHBS were checked by the cytotoxicity test, inhibition test of EAC-resetting and inhibition of PT'VM responses. Raji cells of Burkitt's lymphoma originating from B-cells and acknowledged as non-immunoglobulin bearing on the cell surface were stained with AHBS according to the immunofluorescent technique. ATOS showed a higher cytotoxic titer against peripheral B-cells and inhibited EAC-rosetting stronger than ASPS did. On the other hand, macrophages in peripheral blood, Kupffer cells in the liver and certain cells of the thymus-medulla were stained with ASPS, but not with ATOS, even after intensively absorbing ASPS with thymocytes.View full abstractDownload PDF (893K) -
NAOKI MITSUHASHI, MINORU KANEKO, M. D.1977 Volume 121 Issue 1 Pages 77-80
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe fetus is known to be bacteria-free and is contaminated with bacteria during birth. We examined drug-resistant bacteria in feces of new-born infants to know the distribution of drug-resistant bacteria in a hospital. Among 76 infants examined, we could isolate drug-resistant strains of bacteria from 65 infants (86%). We collected 110 drug-resistant strains of Escherichia coli, Klebisella pneumoniae, and Pseudomonas aeruginosa, and could isolate 53 strains (48%) carrying R plasmids; they being 33R (TC. CM. SM. SA. APC) and 20R (TC. CM. SM. SA) plasmids. The data indicated that R plasmids with the same resistance patterns were distributed in a hospital, and new-born infants were contaminated with bacteria carrying R plasmids, although the contamination rate of drug-resistant bacteria in the intestinal flora was very low.-View full abstractDownload PDF (222K) -
OTOTAKA HIGASHI, YOKO KIKUCHI1977 Volume 121 Issue 1 Pages 81-84
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe inhibitory effect of vitamin E-nicotinate upon the hydrogen peroxide (H2O2)-induced platelet aggregation was found to be greater than that of either vitamin E alone or the combination of vitamin E and nicotinic acid. Nicotinic acid showed no inhibitory effect. It was suggested that the effect of vitamin E-nicotinate was not due to the additive effect of vitamin E and nicotinic acid produced by hydrolysis, but to the unique and distinctive property of vitamin E-nicotinate itself.View full abstractDownload PDF (237K) -
SEIKI ITO, YUKIO YAMADA, SHIGERU KOBAYASHI1977 Volume 121 Issue 1 Pages 85-90
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSSomatostatin-immunoreactive cells in the canine gut were investigated by an indirect immunofluorescent technique. Somatostatin-positive cells were demonstrated in the neck portion of the pyloric gland and in the bottom of the intestinal crypt, and these cells were distinguished from those in other organs by the presence of a cytoplasmic process reaching the gut lumen (external environment). Possible functional meaning of this cytoplasmic process was discussed.View full abstractDownload PDF (1546K) -
AKIO NAGASAKI, AKIRA ISHIMORI, OSAMU MASAMUNE, SUSUMU YAMAGATA1977 Volume 121 Issue 1 Pages 91-97
Published: 1977
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn this study is intended a measurement of the pressure of the lower esophageal sphincter (LES) in resting state and after various stimuli such as beef soup and AOC-tetrapeptide in cases of healthy subjects and various diseases by means of an infused open-tip method. The following results were obtained: In the cases of esophagitis, the resting LES pressure is lower than in the cases of healthy subjects and after stimulation by beef soup or tetrapeptide the LES pressure is less elevated. Based on these observations, we concluded that there are some factors other than gastrin which make the LES pressure in the cases of esophagitis lower than in controls.View full abstractDownload PDF (439K)
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