The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
A Rapid Fluorometric Assay of Angiotensin-Converting Enzyme
MAKOTO HAYAKARI, YOSHIKAZU KONDO
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Keywords: angiotensin-converting enzyme, angiotensin I, angiotensin II, Fluorescamine
JOURNAL FREE ACCESS

1977 Volume 122 Issue 4 Pages 313-320

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Abstract

A rapid, simple and sensitive method is described for the fluorornetric assay of angiotensin-converting enzyme using Fluorescamine. The critical factors such as optimal pH, incubation time, chloride ion, and inactivation by EDTA and 8 -hydroxyquinoline were examined. The Km value for hippuryl-L-histidyl-L-leucine was 0.5mM. This method was applied to the assay of angiotensin-converting enzyme in the rat serum and the reproducible values were obtained with a 10 μl of the rat serum.

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