抄録
We applied cell electrophoresis to detect the effect of anesthetics on membrane, and clarified the concentration - effect relationship and the term of reversibility. The cell electrophoretic mobility of rat erythrocytes decreased with time of incubation with intravenous anesthetics (thiopental or droperidol) at clinical concentrations and reached a minimum value of 25% reduction in 30 min. The effect changed gradually in the concentration range of 0.5-1000 μg/ml of thiopental, and changed markedly with 0.3-1 μg/ml of droperidol. Washing out of the anesthetics and subsequent incubation in serum-containing medium resulted in the complete recovery in the mobility when 5 μg/ml thiopental or 1 μg/ml droperidol was used. With high concentration of thiopental (100 μg/ml) or droperidol (10 μg/ml), however, unrecovered fraction of cells increased with time of treatment. The critical time for retaining the reversibility was about 2 hr.
