Inhibition of Prolyl Hydroxylase Activity in the Aorta of Rats by Propranolol in Vitro

Abstract

The enzyme of prolyl hydroxylase was prepared from the rat aorta. The prolyl hydroxylase activity was measured by the tritium release assay in vitro. Propranolol at concentrations of 3×10^-6 M, 10^-5 M and 3×10^-5 M inhibited the prolyl hydroxylase activity by 20.6%, 69.8% and 93.7% of control activity, respectively, when the reaction mixtures were incubated for 30min. It is suggested that propranolol may exert some influence on the collagen biosynthesis of the blood vessels.