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Volume 132 (1980)
- Issue 4 Pages 369-
- Issue 3 Pages 245-
- Issue 2 Pages 123-
- Issue 1 Pages 1-
Volume 132, Issue 3
Displaying 1-17 of 17 articles from this issue
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KOROKU HASHIMOTO, SHIGERU MOTOMURA, TOSHIHIKO IIJIMA, NORIO TAIRA1980 Volume 132 Issue 3 Pages 245-259
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSEffects of ouabain on atrioventricular (AV) conduction were studied by using the canine excised, blood-perfused AV node preparations. Ouabain was continuously infused either selectively into the AV node artery (I) or systemically i. v. into the pentobarbital-anesthetized (II) and the pithed donor dog (III). AV conduction in the isolated AV node preparation was interrupted by AV block in the half of I and II and in all of III after prominent prolongation of AV conduction time. In the remaining half of I and II, AV conduction was interrupted by accelerated prematures. The prematures continued to increase. After AV block ventricular rate decreased in the half of I and II and the inherent automaticity was sustained in all of III. PQ interval of the donor dog was interrupted by accelerated prematures in all of II and two thirds of III. In one third of III, AV block was induced. Interrupting doses of ouabain in the isolated AV node preparation of II and III and in the pithed donor dog heart were almost similar, all of which were significantly larger than that in pentobarbital-anesthetized dog heart. It is concluded that ouabain depresses directly AV conduction but this effect was modified by sympathetic nerve activity in the nerve-intact animals and also by inherent characteristics of the AV nodal cells.View full abstractDownload PDF (944K) -
D. A. BABARYKIN, R. L. ROZENTAL, M. YU. VALINIETSE, V. K. BAUMAN, N. A ...1980 Volume 132 Issue 3 Pages 261-266
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSEffects of prednisolone on the biochemical indices of blood serum, ash content in bones, calcium absorption and calcium-binding protein (CaBP) in the intestinal mucosa were studied. The oral administration of prednisolone (10mg/kg) for 2 weeks reduced CaBP content and calcium absorption to the level typical for D-avitaminosis. The oral administration of 1α-hydroxycholecalciferol (390pmoles/day) in combination with prednisolone for a week returned calcium absorption and CaBP to the control value.View full abstractDownload PDF (370K) -
YOSHIKI KURIYA, TETSURO NISHIHIRA, SHOZO MORI, TOSHIO WATANABE, TAIJI ...1980 Volume 132 Issue 3 Pages 267-276
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe mixed lymphocyte tumor cell culture reaction (MLTR) using autochthonous peripheral and lymph node lymphocytes was performed on 21 patients with esophageal cancer. Ten of 16 cases (62%) in peripheral lymphocytes and 9 of 16 cases (56%) in the lymph node lymphocytes showed positive reaction. There was no correlation between stimulation index (SI) and the degree of lymph node metastasis which is one of the determinants of clinical stage (Guide Lines for the Clinical and Pathologic Studies on Carcinoma of the Esophagus, 1976). Many cases showed positive reaction in MLTR even if their tumors were at advanced stage. SI of MLTR on the patients who died of a relapse in a short time within one year after operation was found to be low levels. There was no remarkable correlation between MLTR and PPD skin reaction. The results of MLTR were compared with histologic differentiation grades of tumor tissues; intraepithelial spread, vascular invasion, sinus histiocytosis of the regional lymph nodes, and follicular hyperplasia of the regional lymph nodes. There was a minor correlation between the results of MLTR and sinus histiocytosis of the regional lymph nodes.View full abstractDownload PDF (564K) -
OSAMU NAKAGOMI, NAKAO ISHIDA1980 Volume 132 Issue 3 Pages 277-287
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSLivers from human fetuses between the 16th and 24th weeks of gestation were dissociated by successive dispase and collagenase digestion followed by two cycles of low-speed differential centrifugation. This improved method recovered approximately 1×107 cells (90% hepatocytes and 90% viable cells) from 4g of liver tissue. These hepatocytes were set into primary culture and monolayer granular hepatocytes were obtained within a week. Both albumin and α-fetoprotein production was demonstrated in these granular hepatocytes by the immunoperoxidase method for 2 weeks and α-fetoprotein production in the culture medium occurred for a week by the single radial immunodiffusion method. The morphological features of the granular hepatocytes could be distinguished from those of the other type of epithelial cells with clear cytoplasm. During the cultivation period, gradual changes from granular to clear hepatocytes with high mitotic activity were found.View full abstractDownload PDF (3092K) -
TAKAO KIKUCHI, TAKAYOSHI TOYOTA, EIETSU OUCHI1980 Volume 132 Issue 3 Pages 289-293
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTo study association between juvenile onset diabetes (JOD) and major histocompatibility gene complex, 40 patients with childhood onset diabetes and 120 healthy subjects were typed for HLA. Bw54 was present in 33 percent of the patients with JOD, while it appeared in 8 percent of the controls. Expressed as a relative risk, the antigen Bw54 confers a susceptibility to the development of JOD which is 5.3 times that in the controls. JOD shows a little high degree of association with A9 (78%). However, the A9-antigen is common in the Japanese and appears in 58 percent. Though less striking, the decreased frequency of B12 was 3 percent of JOD, less than 15 percent of the controls (p<0.05). There was no association between Bw54 and JOD with family history of diabetes.View full abstractDownload PDF (274K) -
SHOJI TERUI, HIYOSHIMARU OYAMADA, HIROSHI HASEGAWA1980 Volume 132 Issue 3 Pages 295-304
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSHepatobiliary scintigraphy with 99mTc-PI was performed on 16 patients after various types of hepatectomy for either primary or secondary liver tumor. 99mTc-PI scintigraphy was found very useful, especially in regard to clinical evaluation of indistinct low-uptake areas observed on a 99mTc colloid liver scintigram in the regenerating liver after hepatectomy. Both tests were conducted successively within a couple of weeks. Colloid scintigrams after hepatectomy were divided into two groups according to the sites of indistinct low-uptake areas; one showed them near the resected margin of the regenerating liver (Type I), and the other showed them in both marginal and intrahepatic regions (Type II). Of these 16 patients, 8 showed Type I and 7 showed Type II. The remaining one was excluded for postsurgical complications. By comparing the colloid scintigram with the 99mTc-PI scintigram, in all the cases of Type I indistinct low-uptake areas were found to be the hilum with a shifted common bile duct. On the other hand, in 2 cases of Type II indistinct low-uptake areas were caused by the hilum accompanied by the dilated bile ducts. In the remaining 5, indistinct low-uptake areas were not identified as bile ducts but were confirmed to be a recurrence of the tumor.View full abstractDownload PDF (2751K) -
JUNKO NAKAO, HAJIME ORIMO, HIDEKI ITO1980 Volume 132 Issue 3 Pages 305-312
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSWhile the incidence of glucose intolerance increases with age, the diagnostic criteria of diabetes mellitus in the aged have been controversial. In this study, glucose intolerance in the aged was classified according to the changes in Hb A1 concentrations and plasma glucose levels at the time of 50g oral glucose tolerance test. Ninety-six aged Japanese (60-90 years old, mean 72 years) were studied. Hb A1 was measured by Fast Hb Test System. The results suggested the existence of critical plasma glucose levels for the increment in Hb A1 in the aged, and the critical values were around 120mg/100ml at fasting, and around 240mg/100ml at 60 or 120 min after glucose load. Using these critical plasma glucose levels, glucose intolerance in the aged was classified into two groups, i. e., Group I with fasting plasma glucose below 120mg/100ml and both plasma glucose levels at 60 and 120 min below 240mg/100ml, and Group 2 with fasting plasma glucose above 120mg/100ml and both plasma glucose levels at 60 and 120 min above 240mg/100ml. The incidence of elevated Hb A1 in Group 1, 4.3%, was significantly lower than 94. 1% in Group 2, and insulinogenie index at 30 min in Group 1, 0.74, was significantly higher than 0.07 in Group 2. In conclusion, it was proposed that subjects in Group 2 represent diabetes mellitus, while subjects in Group 1 might not be of pathological glucose intolerance in the aged.View full abstractDownload PDF (471K) -
MASAAKI KUWAHARA1980 Volume 132 Issue 3 Pages 313-321
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSDimethylphenyl-piperazinium (DMPP) caused a relaxation of a guinea pig urinary bladder in vitro when an intraluminal pressure of bladder had been increased by physo-stigmine or histamine. The relaxing effect of DMPP was also observed in the contractions elicited by electrical stimulations. It was not blocked by hexamethonium, phenoxybenzamine, phentolamine, propranolol or tetrodotoxin. ATP and amines (such as glutamic acid, glycine, GABA, 5-HT and histamine) caused no relaxing effect on the physostigmine-induced contraction. The results indicate that the DMPP-induced relaxation appears to be a direct action of DMPP on the smooth muscle of the bladder and to differ from its adrenergic transmitter releasing or atropine-like action.View full abstractDownload PDF (521K) -
KUNIO SUWA, HIDEO YAMAMURA1980 Volume 132 Issue 3 Pages 323-328
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSFrom the data of the vasomotor waves (VMW) previously reported, we attempted to retrieve information of the gain and the dead-time of the feedback circuit involved. A mathematical analysis revealed a certain relationship between the gain and the dead-time for the VMW to occur in three models. Combining our result with the data of animal experiments obtained by other researchers, we could exclude one of three models, thereby setting the area of existence of gain-delay value. When we apply a value of 3.4 sec for dead-time, as extrapolated from the animal data, the static gain of the system is between 2.2 and 4.0. Contrary to those observed in animal experiments and to our own original speculation, the VMW in man during cardiopulmonary bypass is likely caused via baroreceptor (carotid sinus) reflex rather than through cerebral ischemic response.View full abstractDownload PDF (364K) -
RYOJI MINAMI, FUKUMI NAKAMURA, TOORU KUDOH, KAZUHIKO OYANAGI, TOORU NA ...1980 Volume 132 Issue 3 Pages 329-335
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTotal hexosaminidase (Hex) in the liver obtained from Hurler and Hunter syndromes displayed about ten times higher activity than that in control liver and moreover, its thermostability was significantly increased. On DEAE cellulose column chromatography, Hex A in the liver of those patients was eluted at much higher salt concentration than that required for elution of Hex A from control liver. There was no difference in elution patterns of Sephadex G-200 and Concanavalin A-Sepharose column chromatographies between the altered Hex A and control Hex A. It appears that the glycosaminoglycans accumulating in the liver of those patients are partly bound to Hex A, and that this binding may cause some change of its properties such as ionic charge and thermostability.View full abstractDownload PDF (939K) -
HIROSHI MUNAKATA, ZENSAKU YOSIZAWA1980 Volume 132 Issue 3 Pages 337-340
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe defatted rabbit skin was extracted with 0.5M LaCl3, and the extract was dialyzed exhaustively against distilled water. The precipitate formed during dialysis was dissolved in 28mM EDTA (ethylenediamine tetraacetate) (pH 7.0). Fr. A was obtained from the solution by precipitation with ethanol in the presence of sodium acetate. The results of gel filtration on Sepharose 4B, electrophoresis on cellulose acetate membrane before and after digestion with Streptomyces hyaluronidase, and analytical data indicated that Fr. A was hyaluronic acid with high molecular weight. The present observation together with previous findings suggest that the binding status of proteoglycans in the skin differs significantly from that in cartilages.View full abstractDownload PDF (252K) -
SHINRI TAMURA, KUNIMI KIKUCHI, HISAKO KIKUCHI, AKIRA HIRAGA, SHIGERU T ...1980 Volume 132 Issue 3 Pages 341-348
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSWhen the crude phosphoprotein phosphatase fraction of rat liver cytosol was treated with 80% aqueous ethanol at room temperature, the activity with phosphorylase a as substrate was increased by 110%, but those with glycogen synthase D and phosphohistone were decreased by 53 and 34%, respectively. Chromatography of the ethanol-treated fraction on DE-52 revealed that while phosphoprotein phosphatase IA (Mr=69, 000) remained to exist even though it was reduced, phosphatases IB (Mr=300, 000) and II (Mr=160, 000) were totally replaced by a new phosphatase form with an approximate molecular weight of 35, 000. This low molecular weight form has been designated phosphatase III. When partially purified phosphatases IB and II were separately treated with ethanol, they were converted to phosphatase III. These results suggest that phosphoprotein phosphatases IB and II, but not IA, contain phosphatase III as a subunit. Phosphatases IB and II, however, must differ in structure since “IB to III” is accompanied by an increase in phosphorylase phosphatase activity much greater than that for “II to III”View full abstractDownload PDF (488K) -
MASAHIRO MAKI, KENJI SOGA, HARUO SEKI1980 Volume 132 Issue 3 Pages 349-354
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIt has been a general concept that fibrinolytic activity is diminished during pregnancy. However, increase in FDP (fibrin/fibrinogen degradation products by plasmin digestion) which indicates accelerated fibrinolytic activity is also observed during pregnancy. To clarify these conflicting findings, α-2-plasmin inhibitor and FDP, sensitive indicators of fibrinolytic activity, were studied during pregnancy. Decrease in plasmin inhibitor and increase in FDP were found during pregnancy suggesting that fibrinolytic activity is accelerated during pregnancy. Since FDP level was higher in blood samples from the uterine vein than those from the artery, the accelerated fibrinolysis characterized by decrease in plasmin inhibitor and increased FDP, might be reflection of local event. On the other hand, systemic blood remains still hypofibrinolytic in normal pregnant condition.View full abstractDownload PDF (265K) -
MITSUYUKI TERASHIMA, NORITOSHI TAKEICHI, KAZUFUMI SUZUKI, TOSHIYUKI IT ...1980 Volume 132 Issue 3 Pages 355-361
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSImmunization with xenogenized tumor cells, tumor cells infected with murine leukemia viruses, produced strong antitumor immunity. In this study, the immunogenicity of xenogenized tumor cells was compared in cyclophosphamidetreated and non-treated rats. CY treatment 3 days before immunization with xenogenized tumor cells enhanced antitumor cell-mediated immunity and antibody responses. However, CY treatment after immunization had no effect. Transfer of thymus cells from normal rats before the tumor challenge abolished the CY induced augmenting effect. From these results, it is suggested that the elimination of precursors of suppressor T-cells by CY treatment results in enhanced immunogenicity of xenogenized tumor cells.View full abstractDownload PDF (422K) -
TOSHIKAZU GOTO, KEISHI ABE, YOICHI OTSUKA, TORU ITOH, YUTAKA IMAI, MAK ...1980 Volume 132 Issue 3 Pages 363-364
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe changes of active and inactive renin in plasma after the oral administration of 25mg or 50mg of SQ 14225 (Captopril) were studied in 29 hypertensive patients. Inactive renin was calculated as plasma renin activity (PRA) after cold storage minus PRA before cold storage. The patients were divided into 2 groups, responders and nonresponders, according to the response of active renin to Captopril. In 9 responders, the active renin increased markedly while the inactive renin decreased. On the other hand, in 20 non-responders, both renin activities increased only slightly. These results suggest that inactive renin may be converted in vivo to active renin by Captopril.View full abstractDownload PDF (125K) -
KAZUHIKO IWATSUKI1980 Volume 132 Issue 3 Pages 365-366
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe enzyme of prolyl hydroxylase was prepared from the rat aorta. The prolyl hydroxylase activity was measured by the tritium release assay in vitro. Propranolol at concentrations of 3×10-6 M, 10-5 M and 3×10-5 M inhibited the prolyl hydroxylase activity by 20.6%, 69.8% and 93.7% of control activity, respectively, when the reaction mixtures were incubated for 30min. It is suggested that propranolol may exert some influence on the collagen biosynthesis of the blood vessels.View full abstractDownload PDF (95K) -
SHOZO NAKAMURA, YOSHIAKI TAKEZAWA, TOSHIYUKI MAEDA1980 Volume 132 Issue 3 Pages 367-368
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSSubtypes of hepatitis B surface antigen (HBsAg) were studied in 20 asymptomatic HBsAg carriers and 18 HBsAg-seropositive patients with chronic hepatitis or liver cirrhosis. Among them 25 were university students. In the male university students, subtype adw was found to be significantly higher in asymptomatic HBsAg carriers than in patients with chronic hepatitis (p=0.04). The difference in subtypes of HBsAg between male asymptomatic carriers and male patients with liver disease was further confirmed by the combined results of university students and other subjects (p=0.007).View full abstractDownload PDF (146K)
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