The Specificity of Anti-A Agglutinins from Immunized Rabbits and Plant Lectins

Abstract

SAGISAKA, K., IWASA, M., YOKOI, T. and KANEMITSU, K. The Specificity of Anti-A Agglutinins from Immunized Rabbits and Plant Lectins. Tohoku J. exp. Med., 1981, 135 (2), 155-163-A⁺ and A^- rabbits were immunized with human A red cells, saliva of A⁺ or A^- rabbits or human A.Se. A^- rabbits responded well against A.Se saliva and A cells and slightly against A⁺ saliva, whereas A⁺ rabbits produced high-titered antibody against A cells which was abolished by absorption with O cells. Agglutination inhibition tests showed that anti-A from A^- rabbits immunized with A.Se or A cells was not inhibited with A⁺ saliva, and anti-A from rabbits immunized with A cells was not absorbed with A.Se saliva. Hydroxylapatite chromatography separated each of A.Se and A⁺ saliva into three fractions of which the 1st and 3rd fractions of A.Se and only the 3rd of A⁺ had A activity. The 3rd fraction of A.Se and A⁺ did not inhibit anti-A from any rabbits immunized but the 1st of A.Se inhibited anti-A from all rabbits except for A⁺ immunized with A cells. The 1st and 2nd fractions reacted slightly with anti-A from A^- immunized with A⁺ and from A⁺ immunized with A cells. D. biflorus anti-A distinguished human A from rabbit one but Mushroom anti-A could not. These results suggested qualitative differences between human and rabbit saliva A antigens.