An Esterase Isozyme in Non-Hemoglobin Proteins of Cell Lysate Reacting with Mushroom Lectin
1982 Volume 136 Issue 1 Pages 53-59
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1982 Volume 136 Issue 1 Pages 53-59
IWASA, M., KANEMITSU, K. and SAGISAKA, K. An Esterase Isozyme in Non-Hemoglobin Proteins of Cell Lysate Reacting with Mushroom Lectin. Tohoku J. exp. Med., 1982, 136 (1), 53-59-Of 20 kinds of plant lectins examined, agglutinin activity of only mushroom lectin was inhibited with non-hemoglobin proteins of the red cell lysate. Chromatography of non-hemoglobin proteins on DEAE-cellulose and on hydroxylapatite resulted in isolating a protein which gave a single precipitation line with esterase activity in immunoelectrophoresis. SDS-polyacrylamide gel disc electrophoresis demonstrated that the protein consisted of two subunits molecular weight of which was approximately 100, 000 each.
