MATSUNO, S., KOBARI, M., MATSUDA, Y. and SATO, T. Diagnosis ofCarcinoma of the Pancreas by Assay of Immunosuppressive Acidic Protein. Tohoku J. exp. Med., 1982, 136 (1), 1-10-To establish a procedure for screening of pancreatic cancer, we assayed the serum level of immunosuppressive acidic protein (IAP) in patients with malignant and benign pancreatic diseases and compared it with the serum level of carcinoembryonic antigen (CEA). In 96% of 46 pancreatic cancer patients, the serum IAP level averaged 928μg/ml; while 500μg/ml or below was obtained as the normal value in 211 healthy adults. The serum IAP level may be elevated in the presence of non-malignant, inflammatory pancreatic diseases. For the differential diagnosis of pancreatic cancer, therefore, other laboratory data need to be taken into account. The serum IAP level was correlated with the progression of pancreatic cancer and may provide a means to determine whether tumor resection was radical or admitting of recurrence. The serum IAP level in samples obtained from different sites of the portal system did not reflect tumor location in patients with pancreatic cancer.
MATSUNO, S., MIYASHITA, E., EJIRI, T. and SATO, T. Zinc and MagnesiumOutput in Pancreatic Juice after Pancreaticoduodenectomy. Tohoku J. exp. Med., 1982, 136 (1), 11-22-Determinations were made of the Zn and Mg contents of pancreatic juice in 12 cases undergoing external pancreatic duct drainage following pan creaticoduodenectomy in order to investigate the output and excretion pattern of trace elements in pancreatic juice and also to study their relations to pancreatic enzyme secretion and fibrosis of the pancreas. The results indicate that the output of Zn in pancreatic juice ran parallel with amylase activity and was closely related to secretion of pancreatic enzymes. Relatively smaller variations were noted of the Mg content in pancreatic juice during the postoperative course, with the pattern of Mg excretion in pancreatic juice being dependent upon the volume of pancreatic juice secreted. The output of both Zn and Mg in pancreatic juice correlated with the volume of the residual pancreatic parenchyma. The amylase/Zn concentration ratio of pancreatic juice remained stationary postoperatively in all cases and was shown to have a negative correlation with the pancreatic parenchymal ratio. The findings suggest that the amylase/Zn concentration ratio can be a reliable indication of the degree of pancreatic fibrosis or of pancreatic exocrine function.
ARAKI, H., MISHINA, T., MIYAKODA, K., FUJIWARA, T. and KOBAYASHI, T. Cytosine Arabinoside Bladder Instillation Therapy for Bladder Tumors. Tohoku J. exp. Med., 1982, 136 (1), 23-28- Bladder instillation of cytosine arabinoside (CA), 400mg in 20ml sterilized distilled water, was performed in 30 cases of bladder tumors. The instillation was done everyday to inpatients or 3 times a week to outpatients, for a total of 20 procedures. The total effective rate was 33%. Although the effectiveness of CA bladder instillation was less than that of mitomycin C and adriamycin, the combination of CA and these drugs might be advisable. Neither general nor local side effect was observed.
MURANO, S., SHIRAI, K., MATSUOKA, N., MORISAKI, N., SASAKI, N., SHINOMIYA, M., MIZOBUCHI, M., SAITO, Y. and KUMAGAI, A. Effect of Tocopherol Deficiencyon Lipid Metabolism in Arterial Wall of Spontaneously Hypertensive Rats onNormal and High Cholesterol Diets. Tohoku J. exp. Med., 1982, 136 (1), 29-34 Lipid metabolism in the arterial wall of spontaneously hypertensive rats (SHR) fed on tocopherol-deficient diet, high-cholesterol diet or both was studied. Serum tocopherol was greatly decreased in tocopherol-deficient SHR. Lipoperoxide, determined as thiobarbituric acid-reactive substances, was higher in tocopherol-deficient SHR than in normal SHR. Tocopherol-deficient SHR showed a decrease in acid cholesterol esterase activity, but no change in neutral cholesterol esterase, acid and neutral lipase, acyl CoA synthetase, cytidine-diphosphate choline-1-2-diacyl glycerol choline phosphotransferase (CPT) or triglyceride synthesizing activity.
KUGIMIYA, T., FUJISAWA, M., SUWA, K., YAMAMURA, H., MINOHARA, K. and KASAMA, T. Effects of Ortho-Iodo-Sodium Benzoate (OISB) on Oxygen-HemoglobinAffinity and Its Relation to Plasma Concentrations in Dogs. Tohoku J. exp. Med., 1982, 136 (1), 35-41-Effects of ortho-iodo-sodium benzoate (OISB) on oxygen-hemoglobin affinity were studied in unanesthetized beagles with simultaneous measurement of its plasma concentrations. Oxygen-hemoglobin affinity changes correlated well with the plasma concentrations of the drug. Intravenous administration of 100mg/kg of OISB increased P50 significantly from the control value of 28.6±0.8mmHg to 29.5±0.7mmHg and 30.0±0.5mmHg, 5min and 30min after the infusion, respectively. P50 gradually decreased thereafter, and after 1hr period, elevation was no longer significant. Administration of 200mg/kg of OISB caused a significant increase in P50 from the control of 29.3±0.3 mmHg to 30.5±0.4mmHg, 31.1±0.3mmHg, 31.1±0.3mmHg and 30.5±0.5 mmHg, at 5min, 30min, 1hr and 3hr, respectively. P50 elevation declined thereafter to control levels. Plasma concentrations of the drug reached its highest level 5min after the administration and declined gradually thereafter. All significant elevations of P50 were accompanied by the plasma concentrations of the drug more than 200μg/ml. Biological half-life of the drug was found to be about 5.5hr.
MINAMI, R. and NAKAO, T. α-D-Mannosidase in the Liver and Brain fromPatients with I-Cell Disease. Tohoku J. exp. Med., 1982, 136 (1), 43-51-The properties of α-D-mannosidase in the liver and brain from patients with I-cell disease were investigated, using 4-methylumbelliferyl-α-D-mannopyranoside as a substrate. A combination of chromatographies on DEAE-cellulose, concanavalin A-Sepharose and Sephacryl S-300 showed that intermediate α-D-mannosidae was present in the liver and brain from I-cell disease as well as control liver and brain, although an abnormal distribution of intermediate and ac idic α-D-mannosidases occurred in the tissues from I-cell disease. These results suggested that a defect of intermediate α-D-mannosidase is unlikely to be the primary defect responsible for I-cell disease.-intermediate α
IWASA, M., KANEMITSU, K. and SAGISAKA, K. An Esterase Isozyme in Non-Hemoglobin Proteins of Cell Lysate Reacting with Mushroom Lectin. Tohoku J. exp. Med., 1982, 136 (1), 53-59-Of 20 kinds of plant lectins examined, agglutinin activity of only mushroom lectin was inhibited with non-hemoglobin proteins of the red cell lysate. Chromatography of non-hemoglobin proteins on DEAE-cellulose and on hydroxylapatite resulted in isolating a protein which gave a single precipitation line with esterase activity in immunoelectrophoresis. SDS-polyacrylamide gel disc electrophoresis demonstrated that the protein consisted of two subunits molecular weight of which was approximately 100, 000 each.
KIMURA, A., HAYASHI, S., KOSEKI, M. and TSURUMI, K. Characteristics ofUrinary Glycosaminoglycans Excreted by a Patient with the Hurler-Scheie CompoundSyndrome. Tohoku J. exp. Med., 1982, 136 (1), 61-66 Glycosaminoglycan isolated from urine of a patient with the Hurler-Scheie compound syndrome consisted of dermatan sulfate (60%), heparan sulfate (34%) and chondroitin sulfate (6%). About 60% of both dermatan and chondroitin sulfates had molecular weight 8, 000-10, 000, while 95% of the heparan sulfate had molecular weight less than 6, 000. The total sulfate content of the glycosaminoglycans increased with decrease in molecular weight. N-sulfate content in the heparan sulfate, however, had no relation to molecular weight, and was 0.33 mole per mole of glucosamine on the average. About 70% of the heparan sulfate with the lowest molecular weight (1, 500) were composed of three repeating disaccharide units of heparan sulfate and two acetyl, one N-sulfate and three O-sulfate groups linked to the units. The dermatan sulfate contained 1.0-1.2 moles of sulfate per mole of galactosamine. Of the excess sulfate 45-65% were bound to iduronate residues and the rest to C-6 of N-acetylgalactosamine 4-sulfate residues. Most of the dermatan sulfate (83.2-100%) had nonsulfated iduronic acid at the non-reducing end. This finding is consistent with the defect of iduronidase in this disease.
SATO, H., ARAI, H., NAGAI, H., ITO, M., SATOH, K., KUMANO, N., MOTOMIYA, M. and KONNO, K. Evidence for Host-Mediated Antitumor Activity in the Treatmentof Experimental Tumor with a Hot Water Extract from BCG. Tohoku J. exp. Med., 1982, 136 (1), 67-74 - Studies were undertaken for the evaluation of the mechanism responsible for antitumor effects of hot water extract (HWE) of BCG on murine ascites tumor cells, a) The viability of tumor cells has remained unchanged after incubation of tumor cells in the medium containing HWE. b) There was no difference in survival time between mice which had received tumor cells treated as in (a) and the control mice. c) Silica, carragheenan and cortisone acetate reduced the antitumor effect of HWE in ddY mice and C3H/He mice which had been inoculated with Sarcoma 180 cells or MH134 cells. d) The growth of tumor cells was retarded when they were inoculated subcutaneously together with peritoneal cells from HWE-injected mice. These results indicate the participation of host-mediated antitumor activity when neoplasms were treated with HWE.
WATANABA, H., KAWAI, K, and CHIBA, S. Frequency-Force Relationship in DogAtrial Muscle as Modified by Acetylcholine. Tohoku J. exp. Med., 1982, 136 (1), 75-78 The effects of acetylcholine on the frequency-force relationship in isolated dog atria were investigated in a frequency range of 2-3.5Hz. The atria were suspended in a bath and perfused with arterial blood from a carotid artery of a heparinized donor dog. The frequency-force relationship was uniformly and dose-dependently suppressed by infusion of acetylcholin in 3 different doses at the frequency range of 2-3.5Hz, and acetylcholine did not induce a negative staircase.
HORI, Y. and NAKAYAMA, T. Temperature Sensitivity of the Preoptic andAnterior Hypothalamic Neurons in Organ Culture. Tohoku J. exp. Med., 1982, 136 (1), 79-87 Single unit activities were recorded from the preoptic and anterior hypothalamic (POAH) explants obtained from newborn mice brain to study neural temperature sensitivities in the period of 10-34 days culture. The firing rate of recorded units was less than 13 impulses per sec at 35°C. Fifteen warm sensitive, 2 cold sensitive and 37 thermally insensitive units were obtained. The temperature sensitivities of the cultured POAH neurons were similar to those of in vivo neurons, i.e. the discharge frequencies changed smoothly in proportion to 1-5°C changes in local temperature. The thermal responses of warm sensitive units increased after 18 days and decreased over 30 days in vitro. Two warm sensitive neurons were identified and observed under the optical microscope. The cold sensitive units were unable to be recorded until about 20 days in vitro. It became evident that the neural temperature sensitivities of POAH neurons were maintained in an absence of the extrahypothalamic afferents.
OHKAWA, H. Excitatory Junction Potentials Recorded from the Circular SmoothMuscles of the Guinea-Pig Seminal Vesicle. Tohoku J. exp. Med., 1982, 136 (1), 89-102 - Spontaneous excitatory junction potentials (e.j.p.s) from the circular smooth muscle cells of the guinea-pig seminal vesicle were recorded intracellularly. Their mean amplitude and frequency were 2.6mV and 26.6/min, respectively. Occasionally large e.j.p.s were recorded. The circular smooth muscle of the tissue generated the action potential spontaneously, which was accompanied by a twitch-like contraction. The e.j.p.s evoked by field stimulation were also recorded. These potentials in response to the initial several stimuli of a train showed a progressive increase in amplitude. The ratio of the later e.j.p. to the initial e.j.p. ranged from 1.0 to 2.5. The summation of successive e.j.p.s was observed. The rate of depolarization caused by the summation was larger as the frequency of stimulation was higher. The action potential was evoked at a certain level of depolarization due to the summation. In some cells, after reaching the maximum depolarization, the membrane was gradually repolarized during the succesive stimuli. In a cell having such properties the action potential could not be evoked. The spontaneous and evoked e.j.p.s were partially blocked in the presence of phentolamine (10-5g/ml). Tetrodotoxin (10-6g/ml) inhibited the initiation of the evoked e.j.p.s. The evoked e.j.p.s were suppressed by yohimbine (5×10-5g/ml) and phenoxybenzamine (10-5g/ml) but not affected by propranolol (10-5g/ml). These results suggest that the spontaneous e.j.p.s in the circular smooth muscle are induced by the action of neurotransmitter released from the nerve endings in the tissue and that the synapse is the alpha-excitatory adrenergic.
TAKADA, G., OHTAKE, M., MIYABAYASHI, S. and TADA, K. Lymphoblasts andDiagnosis of Pyruvate Carboxylase Deficiency. Tohoku J. exp. Med., 1982, 136 (1), 103-107 - Pyruvate carboxylase activity was measured in phytohemagglutinin-transformed lymphocytes (i.e. lymphoblasts). Mean value±S.D. for 10 controls was 259±38 pmoles/min per mg protein. It was about 7 times higher than that of peripheral leukocytes and a half of cultured skin fibroblasts with similar techniques. Deficient pyruvate carboxylase activity less than 6 pmoles/min per mg protein was demonstrated in the lymphoblasts from a patient with biotin-dependent multiple carboxylase deficiency. It is suggested that lymphoblasts may allow more reliable and ready diagnosis of patients with pyruvate carboxylase deficiency.
ITO, S., MATSUBARA, Y. and SHIBATA, A. Plasma RadioimmunoassayableNeurotensin Responses to the Administration of Various Nutrients. Tohoku J. exp. Med., 1982, 136 (1), 109-110 - In order to elucidate the releasing mechanism of gut neurotensin, plasma radioimmunoassayable neurotensin (RNT) was measured in dogs after intrajejunal administration of various nutrients. Transient andslight increases of plasma RNT were found after administration of glucose and saturated fatty acid. In contrast, remarkably raised plasma RNT levels were seen after administration of an amino acid mixture and of unsaturated fatty acid. The findings suggested that gut neurotensin may play roles in digestion and adsorption of amino acid and some fatty acids.
NAGAI, A., TAKIZAWA, T., KONNO, K, and KAWAKAMI, M. A New SimpleMethod to Detect an Air-Leaking Lung Field in Pneumothorax by a FlexibleBronchofiberscope. Tohoku J. exp. Med., 1982, 136 (1), 111-112 - A new simple method was designed to detect an air-leaking lung field in refractory pneumothorax. A constant volume of 100% helium gas was introduced into each lobe bronchus of two patients with pneumothorax via a flexible bronchofiberscope. In both patients, only when helium gas was introduced into the communicating bronchus with a ruptured emphysematous bullae, a high concentration of helium was detected in the intrathoracic gas. Thoracotomy has been used for patients with pneumothorax in which an air-leakage has persistently as well as recurrently continued, but this therapeutical approach has been frequently limited by a patient-age and an underlying disease. An exact detecting of the site of air leakage makes much expectant of an effectiveness of the outlet-closing treatment under a thoracoscope and finally leads to a new transbronchial treatment of pneumothorax.