Two Non-T/Non-B Leukemia Cell Lines (THP-3-1 and THP-3-2) Established from a Patient at Different Stages of the Disease

Abstract

TSUCHIYA, S., KOBAYASHI, Y., YAMAGUCHI, Y., IMAIZUMI, M., MINEGISHI, M., KONNO, T. and TADA, K. Two Non-T/Non-B Leukemia Cell Lines (THP-3-1 and THP-3-2) Established from a Patient at Different Stages of the Disease. Tohoku J. exp. Med., 1983, 140 (4), 443-444 - Two leukemia cell lines of different phenotypic expression were established from a single patient with non-T/non-B acute lymphocytic leukemia (ALL); one (THP-3-1) was derived from the peripheral blood taken on admission before chemotherapy and the other THP-3-2) during the terminal stage. Both THP-3-1 and THP-3-2 cells were positive for terminal deoxynucleotidyl transferase (TdT) and Ia-like antigen but negative for surface immunoglobulins (Ig), nuclear antigen of Epstein-Barr virus (EBNA) and receptors for sheep erythrocytes. However, there was a difference in the expression of common ALL antigen (J5) between the two cell lines. J5⁺ cells were found in only 5% of THP-3-1 and in 96% of THP-3-2.