Glycosaminoglycans from Renal Brush Border Membranes of Rabbits

Abstract

MUNAKATA, H., ISEMURA, M., AIKAWA, J., KODAMA, C. and YOSIZAWA, Z. Glycosaminoglycans from Renal Brush Border Membranes of Rabbits. Tohoku J. exp. Med., 1985, 145 (4), 353-358-Brush border membranes were prepared from the rabbit kidney cortex by sucrose density gradient centrifugation and digested with pronase. A glycosaminoglycan fraction was isolated from the digest by ion-exchange chromatography. Electrophoretogram on cellulose acetate membrane (Separax) of the glycosaminoglycan fraction indicated the presence of two components, which were identified as chondroitin sulfate A/C and dermatan sulfate on the basis of their mobility in Separax electrophoresis and sensitivity to chondroitinase ABC. Neither hyaluronic acid nor heparan sulfate was detected. The results of high-performance liquid chromatography of the pyridylamino derivatives of unsaturated disaccharides in the digests with chondroitinase AC and ABC of the glycosaminoglycan fraction indicated that the ratio of chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate was 5.5:1.0:9.1