Complementation Analysis of β-Galactosidase Deficiency by Means of Histochemical Method

Abstract

MINAMI, R., WAGATSUMA, K., ISHIKAWA, Y., WATANABE, M., KUSANO, T., NAKAO, T. and TSUGAWA, S. Complementation Analysis ofβ-Galactosidase Deficiency by Means of Histochemical Method. Tohoku J. exp. Med., 1985, 146(2), 181-187-Cultured skin fibroblasts from 4 β-galactosidase-deficient patients with different clinical features were hybridized andβ-galactosidase activities were measured using 4-methylumbelliferyl (4MU)-derivate and by means of indigogenic method. Compared with the assays of β-galactosidase activity using 4MU-derivate, more clear-cut evidence was obtained in the indigogenic method for a judgement of complementation.